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GE HEALTHCARE AKTAprime plus Quick Reference Instructions

Also see for AKTAprime plus: Operating instructions

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Contents
  1. System Preparation
  2. Buffer exchange on HiTrap Desalting
  3. Buffer exchange on HiPrep 26/10 Desalting
  4. Histidine-tagged protein purification step elution
  5. Histidine-tagged protein purification gradient elution
  6. IMAC purification - any metal
  7. On-column refolding
  8. GST-tagged protein purification
  9. Strep(II)-tagged protein purification
  10. MBP-tagged protein purification
  11. MAb purification, step elution
  12. MAb purification, gradient elution
  13. IgM purification
  14. Albumin removal
  15. Removal of trypsin-like serine proteases
  16. Anion exchange
  17. Cation exchange
  18. Method Templates value table
GE HEALTHCARE AKTAprime plus Quick Reference Instructions Manual pdf 1 page image
ÄKTAprime plusGE HealthcareCue Cards ContentsSystem Preparation 2Buffer exchange on HiTrap Desalting 4Buffer exchange on HiPrep 26/10 Desalting 6Histidine-tagged protein purification,step elution 8Histidine-tagged protein purification,gradient elution 10IMAC purification - any metal 12On-column refolding 14GST-tagged protein purification 16Strep(II)-tagged protein purification 18MBP-tagged protein purification 20MAb purification, step elution 22MAb purification, gradient elution 24IgM purification 26Albumin removal 28Removal of trypsin-like serine proteases 30Anion exchange 32Cation exchange 34Method Templates value table 36
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This manual is suitable for:
AKTAprime plus
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