Hanna Instruments HI 3864 Instruction Manual

Instruction Manualwww.hannainst.comDear Customer,Thank you for choosing a Hanna Product.Please read the instructions carefully before using thechemical test kit. It will provide you with the necessaryinformation for correct use of the kit.Remove the chemical test kit from the packing material andexamine it carefully to make sure that no damage hasoccurred during shipping. If there is any noticeable dam-age, notify your Dealer or the nearest Hanna officeimmediately.Each kit is supplied with:• HI 3864A-0 Reagent, packets (100 pcs);• HI 3864B-0 Reagent, packets (100 pcs);• 1 checker disc (with 2 discs, LR and HR);• 2 glass vials with caps;• 1 mirror.Note: Any damaged or defective item must be returned inits original packing materials.HI 3864PhenolsTest KitISTR3864 11/99 PRINTED IN ITALYPhenols are widely used in pharmaceuticals, dyes and indica-tors and as general disinfectants. They may occur in householdand industrial wastewaters and in natural waters; they canalso enter potable water supplies and chlorination of suchwaters results in malodorous chlorophenol products that aredetectable from 0.001 mg/L (1 ppb). The HANNA colorimetricmethod determines phenol and all ortho and para substitutedphenols. Since substitution generally lowers the response, thereadable value obtained by this method is the minimumconcentration of phenolic compounds present. Natural waterusually contains less than 0.001 mg/L (ppb) of phenols, butsometimes values up to 0.02 mg/L (20 ppb) also occur.Since the concentration of phenols in wastewater is generallysubjected to biological and chemical degradation, preservesamples in the refrigerator and analyze within 4 hours aftercollection.Note: mg/L is equivalent to ppm (parts per million), ppb areparts per billion.SPECIFICATIONSSPECIFICATIONSSPECIFICATIONSSPECIFICATIONSSPECIFICATIONSSIGNIFICANCE AND USESIGNIFICANCE AND USESIGNIFICANCE AND USESIGNIFICANCE AND USESIGNIFICANCE AND USECHEMICAL REACTIONCHEMICAL REACTIONCHEMICAL REACTIONCHEMICAL REACTIONCHEMICAL REACTIONREAD THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT• Verify if the mirror and the LR (0÷1 mg/L) disc arealready pre-installed in the checker. If not, install bothas shown in the figure.INSTRUCTIONSINSTRUCTIONSINSTRUCTIONSINSTRUCTIONSINSTRUCTIONSPhenolic compounds react to form a yellow to orange dye with4-amino antipyrine in alkaline solution buffered at pH about10. The absorbance of this colored product is proportional tothe concentration of phenols present in the aqueous sample.• Fill each glass vial with 10 mL ofsample, up to the mark.• Insert one of them into the left hand opening of thechecker disc. This is the blank.• Add to the otherglass vial 1packet of HI3864A-0 Re-agent. Replacethe cap andmix.• Add 1 packet of HI 3864B-0 Reagent.• Start the waiting time of 10 minutes.Replace the cap andshake the vial until thepowder has totally dis-solved. The solution willturn a color yellow to or-ange. This is the reactedsample.• Remove the cap and in-sert the reacted sampleinto the right hand open-ing of the checker disc.• Hold the checker disc so thata light source illuminates thesamples from the top.• After the 10 minutes havepassed, rotate the disc while looking at the color testwindows and stop when you find the color match. ReadRange 0 to 1.00 mg/L (ppm) as Phenols LR0.5 to 5.0 mg/L (ppm) as Phenols HRSmallest Increment 0.02 mg/L (ppm) Phenols LR0.1 mg/L (ppm) Phenols HRAnalysis Method ColorimetricSample Size 10 mLNumber of Tests 100Case Dimensions 235x175x115 mm (9.2x6.9x4.5")Shipping Weight 573 g (20.2 oz.)the value in the result window and record it in mg/L (orppm) of Phenols.• If the color of the sample is too intense to make a colormatch with the mirror installed, then remove from thechecker disc:i. the glass vialsii. the LR disciii. the mirror and its holder.• Install the HR (0.5÷5 mg/L) disc and replace the glassvials in the checker disc.• Hold the checker disc so that alight source illuminates thesamples from the back windows.• Rotate the disc while looking atthe color test windows and stopwhen you find the color match.Read the value in the resultwindow and record it in mg/L (or ppm) of Phenols.For best results: Intensely colored samples will make the colormatching determination difficult and they should beadequately treated before performing the test. Sus-pended matter in large amounts should be removedby prior filtration.Caution: Ultraviolet radiation may cause fading of colors.When not in use, keep the disc protected from light,in a cool and dry place.The chemicals contained in this kit may be hazardous ifimproperly handled. Read Health and Safety Data Sheetbefore performing this test.HEALTH AND SAFETYHEALTH AND SAFETYHEALTH AND SAFETYHEALTH AND SAFETYHEALTH AND SAFETYREFERENCESREFERENCESREFERENCESREFERENCESREFERENCESStandard Methods for the Examination of Water and Waste-water, 18 th edition, 199210 mL

/ 1

Related manuals for Hanna Instruments HI 3864