Northwest Leica DM6B Quick Start Manual
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Guide for LAS X Navigator (Tile scanning)1. Initial set-up~ Place slide on stage ‘coverslip up’.~ For transmitted light imaging: reference Quick Guide for Bright Field (color) imaging.~ For fluorescent light imaging: reference Quick Guide for Fluorescent imaging.~ Focus on sample. Find cell/region of interest.~ Set imaging settings as required by experiment.2. Setting up Tile Scanning~ Press the Navigator button located near the top of the OpenProjects/Acquisition panel. New window will open.~ In the new window find access to the standard LAS X settings on the left side (A-G). Adjust settings atany time if required while staying in the Navigator mode.~ Press “Carrier (G) > Well Plate > 4 slide” to view screen as 4 slide stage.~ Using mouse wheel, zoom in/zoom out to the field of view of the interest.~ Double clicking by left button of mouse will preview new areas of the slide. Also, Spiral button (H) canbe activated to preview sample by spiraling pattern. Stop it when needed.~ Using one of Shape tools (I) draw border round the region(s) of interest (J). Each selection will be listedin a Task List (K).Leica DM6B WidefieldQuick Start Guide |
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