Sample to InsightJune 2023QIAcube® HTVersion 4.17User ManualFor QIAcube HT and QIAxtractor®

Qiagen QIAcube HT User Manual

Safety Information

Electrical safety

Biological safety

Chemicals

Toxic fumes

Maintenance safety

Symbols on the QIAcube HT

Introduction

General information

Requirements for QIAcube HT users

on the QIAcube HT

General Description

The QIAcube HT

Instrument lid

Pipetting head and robotic arm

Tip chute

Accessory panel

Principles of operation

QIAxtractor instrument

Vacuum control station and waste bottle

Pinch valve

Reagent trough adapters and reagent troughs

Tip disposal box

and user manual

Installation Procedures

AC power connection

Hardware installation

HEPA filter system

Software installation

Connecting the instrument to the laptop

Hardware Wizard

Configuring the COM port

Important notes before first use

QIAcube HT Software

Mode

Starting the QIAcube HT Software

The software workspace

Virtual" mode indicator

Toolbar

Right-hand pane

Menus

Control" menu

Wizards" menu

Appendix F

Help" menu

Workspace items

Plates

Choosing the plate type

Defining favorite plate types

Changing plate function

Tips

Tip properties

Tip availability

Reagents

Configuring reagents

Required reagent volume

Samples and reactions

Sample properties

Reaction properties

Common properties and operations

Entering sample bar codes

Data transfer formats

Importing sample and reaction data

Exporting sample and reaction data

User management

User classes

Enabling access control

Change current user

Revert to first user

Calibrating plate positions

Calibrating plate heights

Calibrating volumes

Calibration management

Reinstating calibration data

Deleting calibration data

Run files

Working with run files

Obtaining run files

Saving run files

Creating a run file with the "Vacuum extraction" wizard

Starting the "Vacuum extraction" wizard

Table setup" step: Configuring the layout

Configuration [1]" step

Configuration [2]" step

Load Pre-Capture Reagent 1" step

Load capture plate" step

Wash step 1 to wash step n" step

Dry sample" step

Elution steps" step

QIAcube HT

safety information

Safety information

general description

General description

QIAcube HT instrument

Feet

HEPA filter

Pipettor head and robotic arm

Vacuum chamber

Internal features of the QIAcube HT

Computer

System delivery

Requirements

Power requirements

Unpacking and installation

Releasing transport locks

Installation of vacuum control station

Installation of pinch valve

UV lamp

Connecting the computer to the QIAcube HT

Uninstalling QIAcube HT Prep Manager Software

Updating QIAcube HT Prep Manager Software

Upgrading from legacy QIAcube HT Software

Installing additional kits

getting started

Getting started

Customizing global settings

Customize user settings

Managing users

Changing password

Managing labware

Assembling the vacuum chamber

Assembly for calibration

Assembly for standard purification protocols

Instrument calibration

Calibrate volumes

Calibration positioning buttons

Calibrate tip racks

Calibrate labware

Prepare worktable for labware calibration

Calibrate X- and Y-axis

Calibrate Z-axis

Saving and importing calibration data

Operating procedures

Setup step

Labware selection step

Select other input labware

Assignment

Assigning samples

Removing assignment

Preparing the worktable

Checking the waste bottle status

Preparing tip chute and tip disposal box

Preparing the vacuum chamber

Loading reagents and samples

Starting a run

Open system test

Pausing/Stopping a run

Vacuum performance check

Run completion

Finishing work and shutting down

Concepts

Experiments and templates

Protection of files

Use of color

Displaying errors and warnings

Entering data

Working with tables

Selecting samples

General elements

Main toolbar

Button bar

File dialog

Scan plate barcode dialog

Home

Experiments

Service

Configuration

Getting help

Working with experiments

QIAxtractor