Pentra 808–6 Pentra 80 - User Manual - RAB108EA2.8. Reagent consumption (ml)Tab. 8–3: Reagent consumptionSTARTUP cycle estimated duration and consumptions are given for one blank cyclecontrol. It could be a maximum of three cycles.2.9. Recommended AnticoagulantThe recommended anticoagulant is K3EDTA, with the proper proportion of blood toanticoagulant as specified by the tube manufacturer. K2EDTA is an acceptablealternative.Cycles Estimatedduration (s)Diluent(ml)Eosinofix(ml)Basolyse II(ml)Cleaner(ml)Lyse(ml)CBC/DIFF 0’45’’ 27.4 1.0 2.0 1.0 0.45CBC 0’45’’ 24.4 - 2.0 1.0 0.45Prime DILUENT 3’00’’ 44 - - - -PrimeEOSINOFIX 1’34’’ 1.6 23.7 - - -PrimeBASOLYSE 2 1’25’’ 1.7 - 23.7 1.0 -Prime CLEANER 1’24’’ 1.7 - - 24.7 -Prime LYSE 1’31’’ 2.7 - - - 8.4Prime ALL 7’13’’ 50.7 24.0 24.0 25.0 8.4STARTUP(1 blank cycle) 2’28’’ 55.2 2.0 3.0 2.0 0.95SHUT DOWN 2’56’’ 32.2 1.0 1.0 14.2 0.49RinseCYTOMETER 1’14’’ 5.0 - - - -AUTOCLEAN 1’33’’ 27.6 1.0 1.0 1.0 0.50MINICLEAN 0’21’’ 10.3 1.0 2.0 1.0 0.33CONCENTRATEDcleaning 4’00’’ 29.6 1.0 1.0 1.0 0.50BACKFLUSH 0’24’’ - - - - -
