6. Allow the cabinet to run at least 3 minutes so that the chambers can purge with HEPA filtered air.7. Wipe down the interior area of the cabinet with a surface disinfectant.Note: Some disinfectants, such as bleach or iodine, may corrode or stain the steel surfaces. If thishappens, thoroughly clean the surfaces afterward with a detergent and rinse with sterile water toprevent corrosion.Loading Materials and Equipment1. Place all materials and equipment needed in the cabinet via the pass-through door. After closing the doorwait 1 minute before opening the inter-chamber door. This will allow the pass-through chamber andtunnel enough time to purge with HEPA filtered air. Important: Make sure to disinfect items beforemoving them from the pass-through chamber to the work chamber.2. Avoid blocking front and rear grills.3. Arrange materials to minimize movement within the cabinet.4. Leave the inter-chamber door closed while working in the work chamber.Recommended Work Techniques1. Perform all work on or over the solid work surface. Avoid working on the grills.2. Keep the viewscreen closed and latches locked.3. Limit opening of pass-through and inter-chamber doors when moving materials or equipment.4. Make sure to wait 1 minute anytime the pass-through door has been open and closed to allow thechamber and tunnel to purge with HEPA filtered air.Unloading and Wipe down1. Close the inter-chamber door before opening the pass-through door when unloading materials andequipment.2. Wipe down the work and pass-through chambers with disinfectant before opening the pass-throughdoor. Never use the cabinet to store supplies or laboratory equipment.3. We recommend that the cabinet be left running continuously to ensure cleanliness. If the user elects toturn the building exhaust fan off at the end of a work session, the work chamber viewscreen and pass-through door should be closed completely and latched.Reacting to SpillsSpills should be cleaned immediately to prevent cross contamination to the work and to avoid any damageto the stainless steel surfaces.It is recommended that the researcher, in coordination with their consulting safety professional, have awritten plan available in case of an accidental exposure or spill. The safety plan should include all of theemergency procedures to be followed in the event of an accident. All employees who use the cabinet shouldbe familiar with the safety plan.DecontaminationIt is recommended that the researcher, in coordination with their consulting safety professional, have awritten decontamination plan available based on what the cabinet is being used for. All employees who usethe cabinet should be trained on how to perform the decontamination procedure.351D700 Rev G 10