Barnstead Thermolyne Corporation Turner SP-870 Operation Manual
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Contents
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17Concentration Mode1. Fill a cuvette with the solvent that will beused to zero the spectrophotometer. This isthe blank (or background) solvent that yoursample is dissolved in.2. Place the cuvette in the cuvette holder in thesample compartment. Close the samplecompartment lid.3. Press (λ nm) button, enter the numericalvalue (330-999) of the wavelength and pressthe ENTER button. If a mistake is made inthe key-in process, press the ESC button toclear the display for a new entry.4. Select Transmittance or Absorbance modeby pressing the (T) or (A) button.5. Zero the instrument using the blank solutionby pressing the (100%T/OA) button. Thedisplay should show:XXX TCALB or XXX ACALBthen should readXXX 100.0T or XXX 0.000A(Transmittance mode) (Absorbance mode)6. Carefully open the sample compartment andremove the cuvette.7. Fill a cuvette with a Standard solution ofknown concentration and place the cuvettein the sample compartment.O PERATION |
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