65. Gently apply pressure to the finger near the first knuckle of the ring or middle finger andengorge the end of the finger with a squeeze and release technique.6. Use the first drop of blood to immediately dose the tip of the eGLU test strip until thechannel is filled and the screen shows a beating heart icon over the glucose strip.7. Wipe away the residual blood with a clean, dry gauze pad and use the second blooddrop to begin the capillary tube collection of 40μL of blood to dose the Lipid Panel teststrip. With a gentle, but firm squeeze near the first knuckle, produce a large drop at thefingerstick site and touch the tip of the capillary blood collector or calibrated pipette tothe drop. With 2 to 3 squeeze-and-release motions, allow the horizontal fill of thecapillary tube without the introduction of bubbles or air spaces. In order to prevent air bubbles or gaps, hold the capillary tube at a slight upwardangle and fill the capillary tube to the black line.8. See section VI to perform the test.B. Venous Whole Blood Samples1. Collect the venous sample in either a green top (Lithium heparin) or lavender top (EDTA)tube.2. Gently mix the tube by inversion 10 times.3. Carefully open the tube and remove a 40μL sample using either a capillary bloodcollector or calibrated pipette.4. See section VI to perform the test.5. Venous samples are not suitable for eGLU testing and are only allowed for PTSreflectance test strips, such as the Lipid Panel test portion of the Smart Bundle pack.C. Interfering SubstancesDopamine and methyldopa decreased the results of all lipid analytes, while causing higherresults for the eGLU testing. Extremely high doses of ascorbic acid (Vitamin C) decreased theresults of all the lipid analytes, while having no significant effect on the eGLU results.IV. Equipment/Materials/ReagentsThe Lipid + eGLU Smart Bundle™ consists of three boxes of Lipid Panel test strips which are self-contained disposable test strips preloaded with the reagents necessary to measure totalcholesterol, HDL cholesterol, and triglycerides, as well as one box of eGLU™ test strips preloadedwith the reagents necessary to measure glucose. Calculated LDL results are available forsamples with total cholesterol and HDL cholesterol within the measuring range of the analyzer,and triglycerides levels below 400 mg/dL.