Using a flow cellC A U T I O NDo not use a flow cell with flammable samples or those that containhydrocarbons, solvents, concentrated acids or concentrated bases that maydamage wetted parts of the cells. Conduct tests before use of flow cells if samplecompatibility is not known.Note: Do not use a high pressure flow cell kit with this instrument.Use a flow cell to increase the speed, accuracy and reproducibility ofmeasurement. The manufacturer especially recommends using a flowcell for low turbidity measurements.Install a flow cell1. Fully clean and assemble the flow cell, tubing and stand. Refer toClean a flow cell assembly on page 28 and the user instructionsprovided with the flow cell.2. Fill the flow cell and tubing with water and make sure that there areno leaks or air bubbles.Note: Air bubbles collect in areas that are not cleaned fully.3. Clean the exterior surface of the flow cell with a soft, lint-free cloth toremove water spots and fingerprints.4. Apply a small bead of silicone oil from the top to the bottom of theflow cell.Note: Use only the provided silicone oil. This silicone oil has the samerefractive index as the flow cell glass and masks minor glass scratches.5. Use the oiling cloth provided to apply the oil equally to the surface ofthe flow cell. Remove the excess oil. Make sure that the flow cell isalmost dry.Note: Put the oiling cloth in a plastic storage bag to keep the cloth clean.6. Install the flow cell in the sample cell compartment.7. Push the inlet and outlet tubes in the slots on the top of theinstrument enclosure so the sample cell cover can be installed. Referto the user instructions.8. Put the flow-cell light cover over the flow cell.Note: The standard sample cell cover of the instrument does not close whenthe flow cell is installed.Clean a flow cell assembly1. Disassemble the flow cell assembly.2. Clean the inside and outside of the glass parts with a laboratoryglass cleaning detergent. Follow with multiple rinses with distilled ordemineralized water.Note: All tubing, flow cells, and caps in the flow cell assembly can also besteam sterilized.3. If measuring low turbidity samples, clean the inside and outside ofthe glass parts with 1:1 hydrochloric acid and rinse multiple timeswith dilution water.4. Fill the sample cell with distilled or demineralized water andimmediately put the caps on the sample cell.5. Clean the inside and outside of the plastic parts and tubing withlaboratory detergent and warm water.Note: At intervals, replace the tubing as contaminants, includingmicrobiological growths, are difficult to remove from the inside surface of thetubing.6. Air dry the parts after cleaning.Flow cell maintenance• Keep all parts of the flow cell assembly clean.• At intervals, replace all the tubing to make sure that the system isclean. Keep the tubing as short as possible to minimize air locking andlag time of sample flow. Locate the instrument as close to the drain aspossible.Flow cell operation• Do not use the flow cell for samples that contain large particles thatmay collect and stop the sample from flowing.• Slowly put the sample down the interior edge of the inlet reservoir toprevent mixing of the sample, which can cause air bubbles. Airbubbles create a false positive interference in a turbiditymeasurement.• If bubbles collect in the flow cell, gently tap the flow cell on a softsurface to remove the bubbles. If bubbles continue to collect in the28 English