Hanna Instruments HI 38075 Instruction Manual

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instruction manual

Hanna Instruments HI 38075 Instruction Manual Manual pdf 1 page image

SPECIFICATIONSSPECIFICATIONSSPECIFICATIONSSPECIFICATIONSSPECIFICATIONSInstruction Manualwww.hannainst.comDear Customer,Thank you for choosing a Hanna Product.Please read the instruction sheet carefully before using thetest kit. It will provide you with the necessary informationfor correct use of the kit. If you need additional information,do not hesitate to e-mail us at tech@hannainst.com.Remove the chemical test kit from the packing material andexamine it carefully to make sure that no damage hasoccurred during shipping. If there is any noticeable dam-age, notify your Dealer or the nearest Hanna officeimmediately.Each kit is supplied with:• HI 93702-0 Reagent, packets (50 pcs);• HI 3856-0 Reagent, packets (50 pcs);• Deionized Water, 1 bottle (230 mL)• 1 long path color comparator cube;• 1 color comparator cube;• 1 plastic test tube (14 mL) with screw cap;• 1 plastic pipette (3 mL).Note: Any damaged or defective item must be returned inits original packing materials.SPECIFICATIONSSPECIFICATIONSSPECIFICATIONSSPECIFICATIONSSPECIFICATIONSHI 38075CopperLow and High RangeTest KitISTR38075 02/00 PRINTED IN ITALYRange 0 to 0.25 mg/L (ppm)0 to 6.0 mg/L (ppm)Smallest Increment 0.05 ppm1.2 ppmAnalysis Method Colorimetric, bicinchoninateSample Size 25 mL2.5 mLNumber of Tests 100 (50 for each range)Case Dimensions 230x59x70 mm (9.0x2.3x2.8")Shipping Weight 555 g (19.6 oz.)SIGNIFICANCE AND USESIGNIFICANCE AND USESIGNIFICANCE AND USESIGNIFICANCE AND USESIGNIFICANCE AND USECHEMICAL REACTIONCHEMICAL REACTIONCHEMICAL REACTIONCHEMICAL REACTIONCHEMICAL REACTIONCopper salts react with bicinchoninate reagent to form apurple product in a neutral buffered condition. The amountof color developed is proportional to the concentration ofcopper present in the aqueous sample.Copper is an essential trace element in human diet (thedaily requirement is around 2.0 mg) and a factor in plantmetabolism. Copper salts are used in water supply systemsto control biological growth in reservoirs. High concentrationson the other hand are toxic and corrosion of copper alloys inpipe fittings may introduce considerable quantities into thewater supplies.Note: mg/L is equivalent to ppm (parts per million).INSTRUCTIONSINSTRUCTIONSINSTRUCTIONSINSTRUCTIONSINSTRUCTIONSREAD THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT• To measure copper in the 0-0.25 range, fill the longpath comparator cube with sample up to the 25 mLmark.The chemicals contained in this kit may be hazardous ifimproperly handled. Read the relevant Health and SafetyData Sheet before performing this test.HEALTH AND SAFETYHEALTH AND SAFETYHEALTH AND SAFETYHEALTH AND SAFETYHEALTH AND SAFETYREFERENCESREFERENCESREFERENCESREFERENCESREFERENCESAdaptation of EPA approved method.• Add 1 packetof HI 3856-0reagent.• Replace the cap and mix byinverting the cube several times.• Wait 45 seconds to allow color todevelop.• Determine whichcolor best matchesthe solution in thecube and readthe result directlyin mg/L (ppm) ofCopper.• It is better tomatch the colorwith a whitesheet placed atabout 10 cm (4") behind the comparator.• To measure copper in the 0-6.0range, use the pipette to fill theplastic test tube with 2.5 mL ofsample, up to the mark.• Add 3.5 mL ofDeionized Water toreach the 6 mLmark. Replace thecap and swirl tomix. This is thediluted sample.• Pour then 5 mL of the di-luted sample from the tubeto the color cube (up to themark).• Add 1 packet of HI 93702-0, replacethe cap and shake well.• Wait 45 seconds toallow color to develop.• Determine which color matches the solution in the vessel.Multiply the result by 2.4 to obtain mg/L (or ppm) ofCopper in the sample.• It is better to match the color with a white sheet placedat about 10 cm (4") behind the comparator.2.5 mL25 mL

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