8Important GuidelinesIntroductionThe important guidelines for operating the ENDURO 250 Volt Power Supply are provided inthis section. We recommend that you carefully review these guidelines before operating theinstrument.ImportantFor best results, do NOT use the ENDURO 250 Volt Power Supply at its maximum electricalload limits. Variations in buffer conditions can result in exceeding the power supply’s maximumvoltage, current, or power output capacity and produce undesirable variations inelectrophoretic separations.General Operating InstructionsFollow the instructions below to operate the ENDURO 250 Volt Power Supply.• Turn on the ENDURO 250 Volt Power Supply using the power switch on the rear of theinstrument. Upon start-up, the current settings appear on the screen.• Use the START/PAUSE and STOP keys and output jacks for applications.• Use the CONSTANT key to set up operation mode.RecommendationThe duration of electrophoresis can be defined in time (hours/minutes). When using this or anyelectrophoresis product, we recommend that you adhere to the protocols given in theelectrophoresis product manuals, and durations, specified in time.ImportantFor best results, follow these important guidelines when running multiple gels andelectrophoresis units concurrently.For example:• Avoid running samples of widely differing salt concentrations or sample buffers at the sametime or on the same gel.• Properly prepare and desalt your samples.Note: Variations in conductivity due to differences in buffer salt concentrations can affect the run of all thesamples run at the same time.