Rainin Pipet Lite Series User Manual

Contents

Rainin Pipet Lite Series User Manual Manual pdf 8 page image

64. Wipe any excess liquid from the outside of the tipwithout touching the orifice.5. To dispense, rest the end of the tip against thevessel wall and press the plunger to the first stop.Hold this position a few seconds, or long enoughfor the liquid column to reach equilibrium again.6. Remove the tip from the receiving vessel withoutblowing out the remaining liquid.7. Return excess sample in the tip to the originalsample container, if desired. Discard the used tip.Pipetting Liquids of Varying DensityPipet-Lite lets you compensate for solutions of densi-ty much different from water, by setting the volumeslightly higher or lower than that required. The com-pensation amount must be determined empirically.For example, if pipetting 10 μL of CsCl solution, youdetermine that the volume delivered is actually 8.5 μL(≥5 samples). Change the volume setting to 11.8 μLand repeat the measurements. If the volumes deliv-ered are still not close enough to 10 μL, make anoth-er slight volume adjustment until the measurementsare as desired.Temperature ConsiderationsWarm or cold liquids can be measured with good pre-cision by using a consistent pipetting rhythm. This willhelp minimize any differences in heating or coolingeffects within the pipette.Use a new tip each time for best accuracy and preci-sion when measuring samples with temperaturesgreatly different from ambient, and do not pre-rinse.As with any air-displacement pipette, you will get bestresults if there is no delay between picking up thesample and dispensing it.AutoclavingAutoclavable parts of Pipet-Lite are the shaft and the tipejector: 121°C, 1 bar, 15-20 minutes.Do not autoclave the complete pipette or anyparts other than the shaft and the tip ejector.