GE Amersham Imager 600 Operating Instructions Manual

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6.2.4 Molecular weight assignmentDescriptionThis is the fourth step in the analysis workflow. The selected image is displayed on thescreen and the button MW is highlighted in the workflow. The options for molecularweight assignment are presented beside the image.Molecular weight is calculated using standard molecular weight (MW) markers.Assign molecular weightThe following illustration and table below describe how to assign molecular weight.Molecular weight calibration can only be performed in single channel view.Note:ActionStepSelect the lane containing the molecular weight marker.1Result: The selected lane appears highlighted.Select the standard molecular weight marker from the drop down menu.2If the correct marker is missing:• Add a missing marker by selecting Add new... button. See instructionsbelow.• Edit a marker by selecting the Edit button. See the instructions below.Deselect any missing bands in the sample marker lane by unchecking rele-vant check boxes.3200 Amersham Imager 600 Operating Instructions 29-0645-17 AD6 Analysis software6.2 Software operation6.2.4 Molecular weight assignment