34“Wrong & clear old calibration”: this message appears as a result of an errone-ous slope condition. If the slope between current calibration point and one of theprevious calibration, that was not overriden in current calibration, exceeds slopewindow (80% to 110%), this message will appear. Also, a CLEAR softkey willappear on the right. Press CLEAR to clear old parameters and continue calibra-tion process or ESC to leave calibration.4.3 DISSOLVED OXYGEN CALIBRATIONIf D.O.% saturation range is calibrated, D.O. concentration range will be cali-brated too, and viceversa.The D.O. % saturation value is the result of a comparison between the concen-tration of oxygen in air (100 % ) and in water, so it is suggested to calibrate theprobe near the area where the measurements will be taken in order to have thebest accuracy.Note also that the D.O. concentration values are based on D.O. % saturation,temperature, salinity and atmospheric pressure so it is recommended to use astandard solution or a reference D.O. meter to compare readings during cali-bration.D.O. % saturation calibration can be performed at 1 or 2 standard points (0 %and 100 %), or at a single custom point (50 to 500 %).D.O. concentration calibration can be performed at a single custom point (4 to50 mg/L).4.3.1 ProcedureAfter choosing the D.O. calibration mode in themain calibration menu, select the type of calibra-tion with the arrow keys and then press OK.D.O. % saturationThis calibration starts by default with 100 %.• Fill the probe beaker with approximately 4 mm(5/32”) distilled water and screw it on the probe.• The message NOT READY appears until thevalue is stabilized.• To change standard calibration point, press CAL.POINT.