Idexx VetTest Operator's Manual

Contents

Idexx VetTest Operator's Manual Manual pdf 42 page image

E–2IDEXX VetTest* Chemistry Analyzer Operator’s ManualDilution ProtocolsThe ability to perform dilutions on the VetTest analyzer allows you to qualify extremelyelevated results.Plasma and Serum DilutionsThe VetTest analyzer supports plasma and serum dilutions in two circumstances:When a test value is outside the linearity range of the VetTest analyzertWhen the sample contains interfering substances (e.g., medications) that cause atnonlinear or invalid resultPlasma and Serum Dilution TipsPerform a dilution only when a test value is accompanied by a greater than symbolt PSEBTIFT POUIFQBUJFOUSFQPSU6TFOPSNBMTBMJOF BTUIFEJMVFOUtFor best results, start with a 1:1 dilution (1 part sample to 1 part saline).tDo not exceed 10 parts saline.tUse an accurate measuring device, such as a calibrated pipette or syringe.tPlasma and Serum Dilution ChartVolumes are for example only. Parts Sample + Parts Saline = Total PartsParts Sample Parts Saline Total Parts (Dilution Factor) Ú- 0 1 (undiluted sample) Ú- Ú- 2 Ú- Ú- 3 Ú- Ú- Ú- Ú- Ú- Ú- Ú- Ú- Ú- Ú- 8 Ú- Ú- Ú- Ú- 10 Ú- Ú- 11Parts Saline: the number entered into the VetTest when running the diluted sampleDilution Factor: the total number of parts in the diluted sample; the VetTest automaticallymultiplies the result by this number to correct for the dilutionPreparing Plasma and Serum DilutionsTo prepare a 1:1 dilution:Accurately measure the desired amount of plasma or serum to be diluted and1.gently transfer it to a sample cup.Accurately measure an equal amount of saline and transfer it to the sample2.collected in step 1.Thoroughly mix the sample and saline.3.Proceed to the analysis (see Running a Dilution on the VetTest Analyzer QBHF&