Labnet ENDURO GEL XL E0160 User Manual

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Labnet ENDURO GEL XL E0160 User Manual Manual pdf 14 page image

11IV. APPENDICESA. Buffers for ElectrophoresisTris Acetate EDTA Buffer (TAE):1X Working Concentration: 10X Stock Solution:40 mM Tris base 48.4 g Tris Base20 mM Glacial Acetic Acid (NaOAc) 16.4 g or 11.42 ml NaOAc2.0 mM EDTA pH 8.3 7.4 g EDTA or 20 ml 0.5 MEDTA (pH 8.0)H2O to 1 literTris Borate EDTA Buffer (TBE):1X Working Concentration: 10X Stock Solution:89 mM Tris Base 108g Tris Base89 mM Boric Acid 55g Boric Acid2.0 mM EDTA pH 8.0 6.72g EDTA or 40ml 0.5M EDTA(pH 8.0)H2O to 1 literRNA electrophoresis Running BufferMOPS Acetate EDTA (MAE):Solutions containing MOPS should be wrapped in aluminum foil and storedat room temperature. The buffer tends to yellow with age. Light yellowbuffer may be used, however, dark yellow solutions should be discarded.1X Working Concentration: 10X Stock Solution:20 mM MOPS (pH 7.0) 41.8 g MOPS8 mM NaOAc 800 ml DEPC treated H2O1 mM EDTA (pH 8.0) adjust pH to 7 with NAOH and add:16.6 ml 3M DEPC-treated NaOAc20.0 ml 0.5 M DEPC-treatedEDTA, pH 8 bring to 1.0 liter andfilter