Labnet ENDURO GEL XL E0160 User Manual

Contents

Labnet ENDURO GEL XL E0160 User Manual Manual pdf 6 page image

3Power SupplyOverall dimension 7.5 × 17.0 × 6.2 cmWeight 410 gInput Voltage AC100 - 240V, 50/60HzOutput Voltage 10 to 150 volts; Constant peakvoltage of 150VOutput Amperage 10 to 400 mAMaximum Wattage 45 WTimer 99 hours 59 min, and continuousmodelSafety Switch Micro-sensor (hall) in the PowerSupply. No output without safety lid,Memory Function Automatic memory (the last used Vand T)III. OPERATING INSTRUCTIONSA. Preparation of the Agarose Gel and Electrophoresis Buffer -DNA1. Select the percentage gel necessary to effectively resolve yoursample, using Table 1 as a guideline.Table 1: Gel Concentrations and Resolving RangesTable taken from Sambrook, J., Fritsch, E.F., & Maniatis, T. (1989)Molecular Cloning, A Laboratory Manual, 1, 6.8 613.2. Weigh an appropriate quantity of agarose (0.3 % means 0.3 g ofagarose per 100 ml of gel volume) and place it into a 250 ml flask.Note a 4mm gel will use 100 mls of agarose solution.3. Make 500 ml of either 1X TAE or 1X TBE electrophoresis buffer(see below).Electrophoresis BuffersThe two most commonly used buffers for horizontalelectrophoresis of double stranded DNA in agarose gels are Tris-Acetate-EDTA (TAE) and Tris-Borate-EDTA (TBE). While theConcentration of Efficient Range ofAgarose in Gel Separation of Linear DNA(% w/V) (Kb)0.3% 5 - 600.6% 1 - 200.7% 0.8 - 100.9% 0.5 - 71.2% 0.4 - 61.5% 0.2 - 32.0% 0.1 - 2