Zeiss LSM 880 Quick Operation Manual
Also see for LSM 880: Operating manualQuick start guideUser guideBasic user's guide
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Instruction LSM880 short 2017-12-26.docx 2017-12-26LSM 880 quick operation manualSwitch on- Make sure room temperature is 22-24°C.- If necessary turn on air condition- In the main switch box turn on the following switches 1 2 3 (do not touch the key)- Turn on the computer and log inUser name: multilabsPassword: 123456Position Objective lens Magni-fication NA coverslip Workingdistance Immersion Parfocal length2 Plan Apochromat x10 0.45 0.17 mm 2 mm Air 45 mm3 Plan Apochromat x20 0.8 0.17 mm 0.55 mm Air 45 mm4 (bombila) W Plan Apochromat x20 1.0 0 1.8 mm Water dipping 75 mm1+ adaptor W Plan Apochromat x40 1.0 0 2.5 mm Water dipping 45 mm1+ adaptor Plan Apochromat x63 1.4 0.17 0.19 mm Oil 45 mmSoftware and acquisition- Log into BookitLab and activate your reservation to start Black Zen 2.3- To acquire images click Start System- In the acquisition tab Laser tabSwitch on the lasers, (The Argon laser should be turned on by Standby ON. Please wait 5' minutes untilthe laser stabilizes, its status should be optimal in the properties tab).- Sample mounting and viewingInsert the slide or dish in the holder.To change objective turn the black wheel to point up to release the objective turret. Turn manually to therequired objective, lower the objective by pointing the black wheel in the objective turret down, (if anobjective needs to be replaced please ask the microscopy staff).In the software go to Locate tab.On the microscope’s right side choose VIS- Transmitted lightClick the transmitted light lamp icon, click ON and set desired brightness (or via the microscope).Set the filter turret position to DIC and the condenser below the stage to II or III according to the objectivelens (see objective lens info on touchpad).Set up Kohler illumination.- Reflected light (fluorescence)Open fluorescence shutterSet the filter turret to the desired position- Image AcquisitionOn the microscope’s right side choose LSMIn Zen, Acquisition tab:Load a saved configuration from the Experiment Manager or open an image and below the image clickReuseSelect Show all tools and open the following tabs: |
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