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Zeiss LSM 880 Operating Manual

Also see for LSM 880: Quick operation manualQuick start guideUser guideBasic user's guide

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Contents
  1. Table Of Contents
  2. Table Of Contents
  3. Table Of Contents
  4. Table Of Contents
  5. Table Of Contents
  6. Table Of Contents
  7. Table Of Contents
  8. PURPOSE
  9. Backup
  10. HARDWARE ASPECTS
  11. Principle of Fluorescence Correlation Spectroscopy
  12. Principle of Airyscanning
  13. Optical Diagram of the LSM 880 (Schematic)
  14. Performance Features of the LSM 880
  15. Microscope Equipment of the LSM 880
  16. Computer Hardware and Software
  17. STARTUP AND SHUTDOWN OF THE SYSTEM
  18. Additional Software Tools
  19. Starting ZEN
  20. Shutdown Procedure
  21. Switching System Power Off
  22. INTRODUCTION TO THE SOFTWARE APPLICATION LAYOUT
  23. Introduction to ZEN
  24. Function Elements
  25. Application Bar
  26. File
  27. Maintain
  28. Camera
  29. Help – About
  30. Main Toolbar
  31. Status Bar
  32. Left Tool Area
  33. Tool Groups and Tools
  34. Context Menu of the Left Tool Area
  35. Center Screen Area
  36. Context Menu of the Center Screen Area
  37. Right Tool Area
  38. LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
  39. Locate Tab
  40. Microscope Control Tool for Axio Observer.Z1
  41. Microscope Control Tool for Axio Imager.Z2
  42. Controls for Axio Examiner
  43. Incubation Tool
  44. Acquisition Tab
  45. Configuration Functions
  46. Smart Setup
  47. Action Buttons
  48. Multidimensional Acquisition Settings
  49. Setup Manager – Laser
  50. Setup Manager – Experiment Designer
  51. Setup Manager – Imaging Setup
  52. Filter Cube Selection
  53. Stage Control
  54. Focus Control
  55. X-Cite Control
  56. Ratio Imaging
  57. Control Elements of the Imaging Setup Tool
  58. Setup Imaging with Airyscan Detector
  59. Acquisition Parameter – Acquisition Mode
  60. Scan Mode
  61. Averaging and Color Depth Selection
  62. HDR(High Dynamic Range)-Imaging
  63. Scan Area Definition
  64. Acquisition Mode Functions for LSM
  65. Acquisition Mode Settings for Airyscan
  66. Acquisition Parameter – Channels
  67. Defining Channels and Tracks
  68. Ratio Channels
  69. Using GaAsP Detectors and the temperature controlled side PMT for Photon Counting Imaging
  70. Channel Settings for Airyscan
  71. Acquisition Parameter – Focus
  72. Acquisition Parameter – Focus Devices and Strategy
  73. Acquisition Parameter – Stage
  74. Acquisition Parameter – Shuttle and Find
  75. Acquisition Parameter – Regions
  76. Multidimensional Acquisition – Z-Stack
  77. Performing a Z-Stack Using First/Last Mode
  78. Performing a Z-Stack Using Center Mode
  79. Performing a Z-Stack Using a Z-Piezo
  80. Optimize Sectioning and Step
  81. Correction
  82. Z-Stack Settings for Airyscan
  83. Multidimensional Acquisition – Tile Scan
  84. Scan Overview Image for Tiling
  85. Multidimensional Acquisition – Time Series
  86. Marker
  87. Start
  88. Position List
  89. Sample Carrier
  90. Setting Individual Offset Values for Positions in Position List Mode
  91. Scan Overview Image
  92. Multidimensional Acquisition – Auto Save
  93. Image Optimization
  94. Defining the Scan Parameters
  95. How to Optimize Image Quality
  96. How to Optimize Imaging with Airyscan
  97. Setup – Laser
  98. Light Path for 2 Channel Module (BiG.2 on DC out port)
  99. NDD Light Path (BiG.2 as NDD)
  100. Light Path for 32 Channel Module
  101. Selection of Laser Lines
  102. Pinhole Adjustment
  103. Setup – Acquisition
  104. Setup – Fit
  105. Defining a New Model
  106. Correlation Options
  107. Model Equations
  108. Definition of a Model
  109. Formula
  110. Setup – Correlator
  111. Navigation – Stage
  112. Navigation – Sample Carrier
  113. Navigation – Focus
  114. Multidimensional Acquisition Tools – Time Series
  115. Multidimensional Acquisition Tools – Positions
  116. Navigation – Incubation
  117. Multidimensional Acquisition Tools – Information on Experiment
  118. Action Buttons for FCS
  119. Method Tool
  120. Activation Tab Area
  121. Count Rate
  122. X-Y-Z Scan
  123. Snap
  124. Taking FCS Measurements
  125. Start and End of a Measurement
  126. Loading Stored Data Files
  127. FCS Correlation Diagram
  128. Image View Controls – Correlation Tab
  129. Image View Controls – Table Tab
  130. Saving Correlation Results
  131. Coincidence
  132. Print
  133. Information
  134. Processing Tab
  135. Processing – Airyscan Processing
  136. Processing – Maximum Intensity Projection
  137. Processing – Color-coded Projection
  138. Processing – Image Calculator
  139. Processing – Average
  140. Processing – Filter
  141. Processing – Linear Unmixing
  142. Processing – Ion Concentration
  143. Single Wavelength Dyes – Offline Calibration
  144. Ratiometric Dyes
  145. Processing – Correlation
  146. Processing – Modify Series
  147. Processing – HDR-imaging
  148. Processing – Stitch
  149. Processing – PALM
  150. PALM – Convert to Image
  151. PALM – Import PALM Molecules
  152. PALM – Localization Precision
  153. PALM – PALM
  154. PALM – Remove PALM Outliers
  155. Processing – Particle Tracking
  156. Processing – Experimental PSF
  157. Processing – Structured Illumination
  158. Structured Illumination – Fourier Transform
  159. Structured Illumination – Structured Illumination
  160. Structured Illumination – TIRF-Filtered
  161. Processing – Channel Alignment
  162. Processing – Lightsheet Processing
  163. Multiview Processing
  164. Online Multiview Processing
  165. Processing – Copy
  166. Processing – ICS
  167. Remove Structure
  168. ICS Correlation
  169. Map Filter
  170. NandB Analysis
  171. Processing – Adjust
  172. Interpolate Brightness and Contrast
  173. Channel Shift
  174. Shading Correction
  175. Maintain Tab
  176. Maintain – Adjust Pinhole and Collimators
  177. Pinholes Section Panel
  178. Collimators Section Panel
  179. How to Adjust the Pinhole and Collimator
  180. Maintain – Objectives
  181. Select Objective
  182. Add Objective
  183. Remove Objective
  184. Focus Speed
  185. Maintain – Filters
  186. Maintain – Airyscan
  187. Options – System Options
  188. System Options – Load Configurations
  189. System Options – Hardware
  190. System Options – Image Display
  191. Options – FCS Options
  192. FCS Options – Measure
  193. FCS Options – Auto Save
  194. CENTER SCREEN AREA / IMAGE CONTAINERS - DISPLAY AND IMAGE ANALYSIS
  195. Container Configuration with the Container Context Menu
  196. Dimensions
  197. Display
  198. Player
  199. Graphics
  200. Preview View
  201. Split View
  202. Gallery View
  203. Airyscan View
  204. Airyscan – View Option Tab Display
  205. Airyscan – View Option Tab Airyscan
  206. Ortho View
  207. Ortho – Select Function
  208. Ortho – Distance Function
  209. Cut View
  210. Shadow Projection
  211. Transparency Render Mode
  212. Maximum Mode
  213. Mixed Render Mode
  214. Clipping Planes
  215. Flying Mode
  216. Series
  217. Interactive Measurements
  218. Settings
  219. Options
  220. D View – Basic
  221. Histogram View
  222. Colocalization View
  223. How a Scatter Diagram is Generated
  224. Quantitative Colocalization Parameters Shown in the Data Table
  225. Overlap Coefficient, Overlap Coefficient after Manders
  226. Shuttle and Find View
  227. Mean of ROI: Additional View Type for Time Series
  228. Kinetic / FRAP View: Additional View Type for Time Series
  229. Lambda Coded: Additional View Types for Lambda Mode
  230. Unmixing View
  231. Automatic Component Extraction
  232. FRET View
  233. Raster Scanning Image Correlation Spectroscopy (RICS)
  234. Access to RICS
  235. Correlation Functionalities
  236. Display Functionalities
  237. Fitting Functionalities
  238. Model Functionalities
  239. Polarization Imaging
  240. Determination of G-factor
  241. Obtaining an Anisotropy Image
  242. Information View
  243. RIGHT TOOL AREA, DATA MANAGEMENT AND STORAGE
  244. ZEN File Browser
  245. Gallery View of the ZEN File Browser
  246. Form View of the ZEN File Browser
  247. Table View of the ZEN File Browser
  248. Images and Documents Panel
  249. Opening of Files via the "Open" Command in the File Menu
  250. Export of Images
  251. Index
Zeiss LSM 880 Operating Manual Manual pdf 1 page image
LSM 880LSM 880 NLOOperating ManualOctober 2014ZEN 2 (black edition)
/ 690
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This manual is suitable for:
LSM 880LSM 880 NLO
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