Zeiss LSM 880 Quick Start Manual

Also see for LSM 880: Operating manual Quick operation manual User guide Basic user's guide

Zeiss LSM 880 Quick Start Manual Manual pdf 1 page image

ZEISS LSM 880 INVERTED (i880) QUICK START GUIDEThe Zeiss LSM880 is a laser scanning confocal microscope with full confocal and multiphoton capability. Theconfocal detectors are spectrally resolved and can be used with either the standard (single photon) excitationlines or the multiphoton excitation lines. The non-descanned (NDD) detectors can only be used with multiphotonexcitation.INSTRUMENT OVERVIEWAND USER RESOURCESFULL TURN ON SEQUENCE (FIRST ONE ON)1) If the computer is off, turn it on and let it boot.2) Turn on the main switch.3) Turn on the Systems/PC switch. (Whentouchscreen has booted, adjust heating andCO2 under Microscope->Inkubation ifapplicable.)4) Turn on the Components switch. Wait for theRTC to boot (see under table).5) Log in to the computer.6) Start Zen and select Start System Button.7) Turn on laser lines you will be using. (If you areusing multiphoton excitation, turn on both lineson even if you are just using one. Note that theAr and multiphoton lines take a while to turn on.You can follow the warm-up progress byselecting the laser and the Laser Propertiesbelow)FULL TURN OFF SEQUENCE (LAST ONE ON)9) Save your data and transfer it to the fileshare.8) Remove your sample and clean off yourobjective. (If using incubation, turn temp andCO2 regulation off and close CO2 tank usingmain gray valve only.)7) Turn off lasers.6) Exit Zen.5) Log out of the computer.4) Turn off the Components switch.3) Turn off the Systems/PC switch.2) Wait a few minutes for the Ar laser to cool.Turn off the main switch.1) Do not turn off computer.PARTIAL TURN OFF SEQUENCE(Someone else is scheduled after your time slot)9) Save your data and transfer it to the fileshare.8) Remove your sample and clean off yourobjective. (If using incubation, turn temp andCO2 regulation off and close CO2 tank usinggray handle only.)7) Turn off lasers.6) Exit Zen.5) Log out of the computer.PARTIAL TURN ON SEQUENCE(Microscope already on)5) Log in to the computer.6) Start Zen and select Start System Button.7) Turn on laser lines you will be using. (If you areusing multiphoton excitation, turn on both lineson even if you are just using one. Note that theAr and multiphoton lines take a while to turn on.You can follow the warm-up progress byselecting the laser and the Laser Propertiesbelow)

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