Baker SterilGARD SG303 Operator's Manual

Contents

Baker SterilGARD SG303 Operator's Manual Manual pdf 18 page image

16passes in or out through the air barrier, until the procedure is completed. Implements should bearranged in the cabinet's work area in logical order so that clean and dirty materials are segregated,preferably on opposite sides of the work area. Blocking the front and rear perforated grilles must beavoided. If wipes or absorbent towels are used on the work surface, be sure to keep them away fromthe grilles.6. After equipment is in place inside the cabinet, adjust the sliding viewscreen so it is open no higherthan the correct access opening height ( 8”). An alarm will signal if that height is exceeded. This isimportant to maintain proper airflow.7. After the cabinet has run for at least three minutes with the window in the proper position, it is readyfor use.Working in the Cabinet1. Hands and arms should be washed thoroughly with germicidal soap both before and after work in thecabinet. Operators are encouraged to wear long-sleeved gowns or lab coats with tight-fitting cuffsand sterile gloves. This minimizes the shedding of skin flora into the work area and protects handsand arms from contamination.2. Perform all work on the depressed area of the solid work surface. Work with a limited number ofslow movements. Since all of the equipment you need is already in the cabinet, it will not benecessary to move your arms in and out through the air barrier.3. Because opening and closing doors in the laboratory causes air disturbance which might interferewith cabinet airflow, this kind of activity should be kept to a minimum while the cabinet is in use.Personnel should also avoid walking by the front of the cabinet while it is in use.4. Avoid using floor-type pipette discard canisters. It is important that your used pipettes be discardedinto a tray or other suitable container inside the cabinet. This reduces the temptation to move in andout of the work area unnecessarily.5. Because of the restricted access, pipetting within the cabinet will require the use of pipetting aids.6. Use good aseptic technique. Procedures done with good technique and proper cabinet methods willnot require the use of a flame. If, however, a safety officer approves the use of flame after evaluatingthe circumstances, then a burner with a pilot light such as the "Touch-O-Matic" should be used.Place it at the rear of the work area where the air turbulence caused by the flame will have the leastpossible effect. Flame disturbs the unidirectional airstream and also contributes to the heat load. Ifthe cabinet blower is unintentionally turned off, the flame could also damage a filter.7. Tubing for a burner within the cabinet should be resistant to cracking or puncture. Material such asTygon tubing is not acceptable for this use.8. Never operate your cabinet while the viewscreen alarm indicator is on. The operating position ofthe sash provides an 8” high access opening, depending on the design set at the factory. Thisrestricted opening permits optimum operating conditions for the cabinet. Because operators will notall be the same height, it is suggested that the operator use a chair which may be adjusted for height.9. After a procedure has been completed, all equipment which has been in contact with the researchagent should be enclosed, and the entire surface decontaminated. Trays of discarded pipettes andglassware should be covered. The cabinet should then be allowed to run for at least three minutes