Baker SterilGARD SG303 Operator's Manual

Contents

Baker SterilGARD SG303 Operator's Manual Manual pdf 19 page image

17with no activity so that the airborne contaminants will be purged from the work area. Next, makesure that all equipment is removed from the cabinet.10. After you have removed all materials, culture apparatus, etc., decontamination of the interiorsurfaces should be repeated. Check the work area carefully for spilled or splashed nutrient whichmight support bacterial growth. Never use the cabinet to store supplies or laboratory equipment.11. We recommend that the cabinet be left running continuously to ensure containment and cleanliness.If the user elects to turn the cabinet off at the end of a work session, the window should be closedcompletely. The sash alarm will be silenced when the window is in the closed position.Reacting to SpillsConsult your safety professional for proper procedures and treatment of the specific agents you plan to use.DecontaminationWhenever maintenance, service or repairs are needed in a contaminated area of your cabinet, the cabinetmust first be decontaminated by an appropriate agent. The National Institute of Health, National CancerInstitute and the Centers for Disease Control have all recommended the use of formaldehyde gas for mostmicrobiological agents. Its application requires individuals who are experienced in the decontamination ofcabinets, since the gas itself is toxic.A good reference for this procedure is NSF/ANSI Standard 49-2002 ANNEX G “RecommendedMicrobiological decontamination Procedure”,NSF International, 789 North Dixboro Road, P.O. Box130140, Ann Arbor, Michigan 48113-0140.Whichever gas you choose, have the proper safety equipment (gas masks, protective clothing, etc.) withineasy reach. In addition, you will want to be sure that the gas you are using will be effective against all of thebiological agents within the cabinet. When you have decided which gas to use, post the antidote to it in avisible and nearby location. The volume of the SG303 cabinet is 30 cubic feet. Provide the correct amountof decontaminating gas for this volume.WARNINGCarcinogens and other toxins present a unique chemical deactivation problem and the standardbiological decontamination will not, of course, be effective against chemicals or other non-biologicalmaterials. With materials of this kind, consult a qualified safety professional.1. Surface-disinfect the inside of the window and all other surfaces on the view screen assembly.2. Multiply the total volume of the cabinet (30 ft3 by .3 gram/ft3 ) to determine the amount ofparaformaldehyde required to decontaminate the cabinet. If the cabinet is vented to the outside you mustconsider the volume of the duct work in the paraformaldehyde calculation.3. Prior to sealing up the cabinet make sure all gas or flammable petcocks are closed and pressure tight.Use a soap bubble solution to make sure there is no leakage. Note: you are creating a confined space.