QIAcube HT User Manual 12/2017 5-276. Repeat the previous step for all volumes from 50 μl to 200 μl.7. Click Save values to save the data or click Cancel to cancel the volume data input and revert any values to theirprevious values.Click the Next button to continue with Tip Rack calibration.Note: The Next button is available only if volume calibration data for the current pipettor head have been saved.5.7.2 Calibration positioning buttonsFor corrections to calibration positions, the software provides several positioning buttons.The positioning buttons are arranged in two groups, one for the Z-axis and one for the XY-axis.