QIAcube HT User Manual 12/2017 5-303. The pipettor head moves to the tip chute, ejects the attached tips and picks up the next available column of tips fromthe tip rack at the selected worktable position.4. Confirm the message with OK. If the tip pickup failed, repeat the tip rack calibration for the selected worktableposition. If the instrument picked up the tips successfully, continue with the next calibration.5.7.4 Calibrate labwareAll labware and adapters must be calibrated before they can be used, to ensure that the pipettor knows their exactpositions on the worktable. In addition, if a certain labware will be used on several worktable positions, it must becalibrated on all of these worktable positions.Labware calibration is performed in the software using a convenient wizard, which guides users through all necessarysteps for the set of labware to be calibrated.The first step of labware calibration is to select the set of labware (or labware/adapter combinations) and the worktablepositions on which the labware will be calibrated. This information is collected in the work list. See Select labware tocalibrate. The calibration wizard will then guide the user through the following calibration steps.The second step is the preparation of the worktable, i.e., placing required tips. See Prepare worktable for labwarecalibration.In several steps, all labware and labware/adapter combinations will be XY-axis calibrated on the worktable positions asdefined in the work list, one step for each labware or labware/adapter combination in a specific worktable position. Theuser will correct the XY-positioning manually and save the calibration data for each step, before continuing with the nextstep. See Calibrate X- and Y-axis.Afterwards, in several steps, all labware and labware/adapter combinations, except for filter plates, will be Z-axiscalibrated, one step for each labware and labware/adapter combination in the work list. For most labware, the softwareprovides an automatic Z-axis calibration function, which should be used whenever possible. However, it is also possibleto decide on each step individually to calibrate the Z-axis manually. Calibration data needs to be saved for each step,before continuing with the next step. See Calibrate Z-axis.Select labware to calibrate5.7.4.1The first step of labware calibration is to select a set of labware along with the adapters and worktable positions onwhich the labware will be calibrated in the work list. Initially, the work list is empty.The labware calibration step will only be available if all previous calibration steps have been previously performed.All labware that has already been calibrated for the currently connected instrument will be listed in the Calibration historytable. Initially, the table is empty.Note: The calibration history will also be empty after the pipettor head has been changed, because calibration data areindividual for an instrument and a pipettor head.
