Varian 4000 MS User Manual

Also see for 4000 MS: Hardware operation manual

Contents

Varian 4000 MS User Manual Manual pdf 19 page image

19Installing a Liquid ReagentTo connect a liquid reagent reservoir to the liquid reagent inlet block, do thefollowing:1. Connect a liquid reagent reservoir containing the chosen liquid to theliquid reagent inlet block.2. Open the CI needle valve 6-7 turns clockwise.3. Click CI Gas Adjustment to open the CI gas valve solenoids.4. Allow the vapor flow from the reservoir to equilibrate. If, after severalminutes, there is not enough CI gas entering the trap, open the needlevalve (clockwise).5. While observing the spectrum, use the Adjust CI Gas, and turn the CIneedle valve to increase or decrease the amount of reagent entering thetrap until the resolution between M and M+1 just starts to degrade.Details for the three most common reagents can be found under the followingsections in the 4000 GC/MS Software Operation Manual: PCI with CH 4, PCI withCH 3CN, and PCI with CH 3OH.Properly Adjusted Methanol Reagent SpectrumProperly Adjusted Acetonitrile Reagent SpectrumNOTE: For best results with acetonitrile, use a filament emission current of atleast 20 μA and maintain at least 50% valley between m/z 41 and m/z 42.