Chapter 5 Analyze the Relative Standard Curve ExperimentView the QC Summary103Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curveand Comparative CT ExperimentsNotesView the QC SummaryThe QC Summary screen displays a list of the 7500 software flags, and includes the flagfrequency and location for the open experiment.About theExampleExperimentIn the standard curve example experiment, you review the QC Summary screen for anyflags generated by the experiment data. In the example experiment, the HIGHSD flagshas been generated for wells: A7, A8, and A9.View the QCSummary1. In the navigation pane, select Analysis QC Summary.Note: If no data are displayed, click Analyze.2. Review the Flags Summary. In the example experiment, three wells are flagged.3. In the Flag Details table, look in the Frequency and Wells columns to determinewhich flags appear in the experiment. In the example experiment, the Frequencycolumn displays 3 for the HIGHSD flag.Note: The Frequency column displays 0 to indicate that the flag does not appear inthe experiment.4. (Optional) Select the HIGHSD flag row to display details about the flag.234
