Chapter 2 Design the Relative Standard Curve ExperimentFinish the Design WizardApplied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curveand Comparative CT Experiments46NotesDesignGuidelinesWhen you design your own relative standard curve experiment:• Select all the materials that you require for your experiment, then add them to yourshopping list.• To access and use the Applied Biosystems Store:– Confirm that your computer has an Internet connection.– Use the following Applied Biosystems-recommended browsers and versions ofAdobe ® Acrobat ® Reader:IMPORTANT! Make sure that cookies and JavaScript are turned on.For MoreInformationFor more information on completing the Materials List screen, open the 7500 SoftwareHelp by clicking or pressing F1.Finish the Design WizardFinish the Design Wizard, review the plate layout, then select an exit option.About theExampleExperimentThe 7500 software automatically selects locations for the wells in the reaction plate. Inthe relative standard curve example experiment:• The wells are arranged as shown below.• The experiment is saved as is and closed.Note: For the example experiment, do not perform the run at this time.DesktopOperatingSystemNetscape®NavigatorMicrosoft ®InternetExplorerMacintosh ®SafariAdobe®Acrobat ®ReaderWindows ®2000/NT/XP/Vistav6.x or later v6.x or later Not applicable v4.0 or laterMacintosh ®OS 9+ or laterNot supported Not supported v2.0.4 or later v4.0 or later
