Chapter 9 Analyze the Comparative CT ExperimentView the Analysis SettingsApplied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curveand Comparative CT Experiments176NotesView the Analysis SettingsThe Analysis Settings dialog box displays the analysis settings for the threshold cycle(CT), flags, relative quantitation, and advanced options. If the default analysis settings inthe 7500 software are not suitable for your experiment, you can change the settings in theAnalysis Settings dialog box, then reanalyze your experiment.About theExampleExperimentIn the comparative CT example experiment, the default analysis settings are used withoutchanges.View the Settings 1. In the navigation pane, select Analysis.2. Click Analysis Settings to open the Analysis Settings dialog box.In the example experiment, the default analysis settings are used from each of thefollowing tabs:• CT Settings• Flag Settings• Relative Quantitation Settings• Advanced Settings3. Click Apply Analysis Settings.
