11-2 Art: 714373-00F Rev. Date: 17-Jan-11STEP ACTION1 Place the cartridge on a flat surface or hold it in a horizontalposition.2 Direct the tip of the syringe, capillary tubeor dispenser into the sample well.3 Dispense sample slowly and steadily untilit reaches the fill mark indicated on thecartridge label. Leave some sample in thesample well.4 Fold the snap closure over thesample well.5 Press the rounded end of theclosure until it snaps into place.Slightly lift finger or thumband ensure that the cartridgeis closed before completelyremoving finger or thumbfrom the closure.EC8+EC8+FILLING AND SEALING CARTRIDGE USING TRANSFER DEVICEProcedureFILLING AND SEALING PT/INR (PROTHROMBIN TIME) CARTRIDGES USING DIRECTFINGERSTICK SAMPLINGSTEP ACTION1. Remove cartridge from foil pouch and place the cartridge on a flatsurface.2. Prepare lancet device and set aside until needed.3. Clean and prepare the finger to be sampled using a 70% aqueoussolution of isopropanol (70% v/v). Allow the finger to drythoroughly before sampling. When disinfecting fingerstick skinpuncture sites, swabs or solutions containing substances other thanisopropanol (e.g. Chlorhexidine Gluconate) are not recommended.Refer to the “Limitations of the i-STATPT/INR Test” section in the PT/INRCartridge and Test Information Sheetfor more information.4. Prick the bottom side of the fingertipwith the lancet device.5. Gently squeeze the finger, developinga hanging drop of blood and performthe test with the first sample of blood.Avoid strong repetitive pressure (“milking”) as it may cause hemolysis ortissue fluid contamination of the specimen.Caution Do not hold cartridge between the fingersif using a syringe with needle to fill.EC8+EC8+PT/INR