Idexx SediVue Dx Operator's Manual

Also see for SediVue Dx: User guide

Idexx SediVue Dx Operator's Manual Manual pdf 12 page image

12Preparing samplesUrine samples that are run on the SediVue Dx* analyzer can be obtainedvia cystocentesis, catheterization, or free catch method. For best results,analyze urine samples within 30 minutes of collection. Otherwise, storethe sample in the refrigerator for up to 2 hours.Note: Storing urine samples in the refrigerator may introduce the in vitroformation of crystals and crystalline debris.If the sample is in a syringe:1. Ensure there is at least 0.5 mL of sample in a 1 mL–5 mL syringe.2. Draw 0.5 mL of air into the syringe.3. Replace the needle with a SediVue* syringe tip.4. Gently invert the syringe 10 times to mix the sample.5. Immediately after inversion, purge all of the air from the syringe and inject 165 μL of urine intothe cartridge fill port.If the sample is in a container:1. Cap the container and invert it 10 times to mix—a well-mixed sample is essential to obtainingaccurate results.2. Immediately after inversion, use the SediVue* Pipette to aspirate165 μL of sample from the bottom third of the container, ensuringthere are no bubbles in the sample, and immediately inject 165 μLof urine into the cartridge fill port.IMPORTANT: Crystals start to settle immediately after inversion.Be sure to aspirate the sample from the bottom third of thesample. Then remove excess urine on the outside of the tip with alint-free wipe.Analyzing a sample1. Initiate the sample run on the IDEXX VetLab Station (for moreinformation, see the IDEXX VetLab Station Operator’s Guide).2. After you’ve confirmed the patient information on the SelectInstruments screen, select the SediVue Dx icon, select the fluidtype if it is not already listed, and then tap Run.3. Once the analyzer has placed a cartridge in the pipetting window,invert the sample 10 times and then immediately inject 165 μL ofurine into the cartridge fill port using either the SediVue Pipette ora syringe with a SediVue Syringe Tip attached.IMPORTANT—When injecting sample into the cartridge:a. Use only IDEXX-supplied pipette tips and syringe tips.They are made specifically for the SediVue Dx analyzerand designed to reduce the risk of spills, leaks, andinappropriately filling the cartridge.b. Hold the pipette or syringe vertically to ensure the sample is injected only into thecartridge. Make sure the pipette/syringe tip is firmly seated into the fill port on thecartridge.Running a Sample on the Analyzer342Stop3