D.8 Connecting the nitrogen exhaust lineRequired materials• Chemical-resistant, powder-free gloves• Utility knife• 12-mm PTFE tubing (included in the Waters Rough Pump Connect Kit)• 12-mm right-angle elbow connectors (included in the Waters Start-Up Kit)Warning: To prevent the nitrogen exhaust from carrying biologically hazardous, toxic, orcorrosive LC solvents, you must use a nitrogen exhaust trap bottle and a laboratory exhaustsystem. The laboratory exhaust system must provide a minimum vacuum of 0.20 kPa (2 mbar,0.03 psi) below atmospheric pressure (negative pressure).Warning: To avoid the buildup of hazardous gases, do not place the nitrogen exhausttrap bottle in an enclosed cabinet.Notice: The instrument requires two separate exhaust systems: one for nitrogen, theother for the backing pump. Vent them to atmosphere through separate exhaust lines.Oil mist can seriously damage the instrument if the nitrogen exhaust line connects withthe backing pump exhaust line. Your warranty does not cover damage caused byrouting exhaust lines incorrectly.To connect the nitrogen exhaust line:1. Locate the exhaust trap bottle in an accessible area below the instrument (see the figure"Exhaust trap bottle" below).Notice: To avoid gas leaks, use the sharp knife to cut the PTFE tubing squarely.2. Cut a length of 12-mm tubing long enough to connect the instrument to the exhaust trapbottle.3. Connect one end of the tubing to the exhaust port on the side of the instrument, and theother end to one of two ports on the exhaust trap bottle.Note: Ensure that there is a negative gradient between the exhaust port and the trapbottle inlet.Notice: To avoid gas leaks, use the sharp knife to cut the PTFE tubing squarely.June 15, 2017, 715003956 Rev. FPage 107