Notice: To avoid causing severe damage to the instrument, use only compatiblesolvents.Starting the instrument entails powering-on the ACQUITY workstation, logging into theworkstation, powering-on the instrument and all other ACQUITY instruments, and starting thesoftware.Requirement: You must power-on and log in to the ACQUITY UPLC workstation first to ensurethat it obtains the IP addresses of the system instruments.To start the instrument:Warning: To avoid igniting flammable solvents, never let the nitrogen supply pressurefall below 600 kPa (6.0 bar, 87 psi).1. On the rear panel, ensure that the nitrogen supply is connected to the instrument's nitrogeninlet connection (see Rear panel connections).Requirement: The nitrogen must be dry and oil-free, with a purity of at least 95%.Regulate the supply at 600 to 690 kPa (6.0 to 6.9 bar, 87 to 100 psi).2. Power-on the workstation and log in.3. Press the power switches on the top left-hand sides of the ACQUITY system modules,including the QDa detector.Result: Each module establishes communications with the workstation.4. Allow approximately five minutes for the instruments to establish communications.Tip: The power and status LEDs show steady green when the instruments haveestablished communications.5. On the workstation, start the software.Result: The instrument performs start-up checks.6. In the software, monitor the Instrument Console for messages and LED indications.2.5 Stop flowWhen the ACQUITY QDa Detector is switched into Standby mode, or a nitrogen gas failure isdetected, the instrument sends a stop-flow signal to the LC. The stop-flow signal switches off allsolvent flow from the LC in order to prevent damage to the ACQUITY QDa Detector. Themessage Stop-flow active appears in the ACQUITY Console when the stop-flow function isactive.Notice: To avoid damage to the instrument, ensure that solvent flow to the instrumentcan be stopped by checking that the Stop Flow external cable is correctly fitted (see theExternal connections appendix).June 15, 2017, 715003956 Rev. FPage 23