33. Select the Configuration you needPick Imaging Configuration from the drop down menuSelect the configuration you needClick the icon to activate your chosen configuration4. Set the Microscope up and find your specimenClick the Ocular button to send the light to your eyes (top right area of theworkspace)This will allow you to check your samples down the microscope before you startlaser scanningIn the Light Path control menu:This opens the transmitted (Brightfield) lightSelect the objective lens that you want to useSelect the appropriate filter (DAPI/FITC/TRITC)Click the fluorescence shutter Opento see fluorescent light

Zeiss LSM 510 Inverted User Manual

Starting the System

Introduction to ZEN – Efficient Navigation

Setting up the microscope

Configuring the beam path and lasers

Scanning an image

Storing and exporting image data

Switching off the system

LSM 510 Inverted

NOTES ON DEVICE SAFETY

Regulations

Notes on Setting up the Microscope System

Notes on Handling the Computer and Data Media

Notes on Care, Maintenance and Service

Notes on Handling the Laser Components

Warning and Information Labels

LSM 510 - SETUP REQUIREMENTS

LSM with Ar UV Laser

LSM prepared for Two Photon Lasers (NLO)

Power Requirements

Phase 1 (LSM)

Physical Dimensions

Dimension of Shipment Crates

Vibrations

LASOS LGK 7786 P / Power supply 7460 A: 543 nm, 1 mW, Laser Class 3 B

Microscopes

Scanning Module

Laser Module VIS (405, 458, 477, 488, 514, 543, 633 nm)

System Overview LSM 510 META

System Overview LSM 510 META - NLO

Notes on setting up the microscope system

Notes on handling the computer and data media

Notes on care, maintenance and service

Notes on handling the laser components

Warning and information labels

LSM with Ar laser (UV) and two microscopes: 450 x 220 cm

Dimension of shipment crates

Laser Specifications

LASOS LGK 7812 ML-1/LGN 7812: 458, 488, 514 nm, 25 mW, laser power class 3 B

Laser Module VIS

Configuration

Quick Guide

Starting up the system

The software will open to the ocular tab

The Acquisition Tab

In the Imaging Setup window

In the Online Acquisition window

Adjusting PMTs

Z series

The Information tab next to the image

APPENDIX B – Parts of the microscope

APPENDIX C – Configuring the Light Path

Setting the microscope

Storing an image