Zeiss LSM 510 Inverted User Manual
Also see for LSM 510 Inverted: Quick guideOperating manualOperating manualUser guideBrief Operating Manual
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45. Start confocal scanningClick LSM button (top right of the workspace)Click the Fast button (found on top left of the workspace) Click Split on the left hand side ofyour image (this splits the imageaccording to channels used)6 . Set the levels for each channelClick the coloured region under each channel (in the panel underneath theimage window) to activate the ‘range indicator’ palette;the images will now go grey or Red and BlueMake sure the channels menu is in your workspace, if not click the right hand side arrow to 'float' the menu outFor each channel and change the following in each to obtain good image:1) Pinhole : click on 1 to make pinhole 1 airy unit (Please note: if you’redoing colocalisation, refer the worksheet on the BALM website)2) Master gain: increase level until you see red, then reduce again untilred just disappears3) Offset: decrease level until you see blue, then increase again until bluejust disappears |
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