Zeiss LSM 510 Inverted User Manual
Also see for LSM 510 Inverted: Quick guideOperating manualOperating manualUser guideBrief Operating Manual
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712. To take a Z stackGo to the Z‐Stack toolbar (found on the left side of the workspace) and drop it downClick on the tick to activate the Z stack menu (you can'tcollect a z stack without this) Its white when activatedChoose if you want to select first and last planes or tocollect data from a central point (BALM staffrecommend select first and last in general)For stacks collected in select First and Last modeEnter your required interval for z stackSelect Keep intervalNote: use Optimal Interval if you want the bestresolved stack (for 3D image generation) Click Fast to start scanning Move to the bottom your sample and hit set last Move to the top of your sample and click set firstClick START to collect the Z stackYou can view the z series (and time series) as it isbeing collected using the Gallery option13. Collecting a time series / timelapseGo to the Time Series icon and drop it down.Make sure you activate the tick (Right hand side)Select the time interval and how many image you can to collect.Click START to start the timelapseNB an icon of a pile will appear below start when a Z series is being collected and a icon of a stopwatchwhen a time series is being collected |
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