Zeiss LSM 510 User Manual

Also see for LSM 510 Inverted: Quick guide Operating manual Operating manual User manual Brief Operating Manual

Contents

Iron Man User Guide v. 1.4 14APPENDIX C – Configuring the Light Path: Only turn on lasers you actually needTo turn on Argon/2 and Diode, first go to Standby and when itsays Ready, then turn it on. Argon/2 output [%] should be 50%(do not go higher). If you have multiple colors, you need to choose if you willbe imaging all channels in a single track or each channel in aseparate track (multi-track).Single track is faster but can lead to bleedthrough of signal.You can do a combination of multi-track and multi-channel –for example, image DAPI and red together in a mult-channeltrack and image green separately in its own track. Load a premade track by clicking on the folder icon. If youwill be using the same track configuration many times, youcan save it here. Note: the loading and saving is done pertrack. This is where you can configure the mirrors and filters inthe light path and check off which channels to image pertrack.Let’s go through an example for imaging a triple labelledsample (DAPI, FITC, Rhodamine) in a single track andmultitrack configuration.