Zeiss LSM 710 Quick Manual

Also see for ConfoCor 3: Quick guide Operating manual

Contents

16 04/2009! For storing a new configuration click andenter a desired name in the first line of the listbox (Fig. 19), then click Ok to store the con-figuration.! For loading an existing configuration clickthen select it from the list box.! For deleting an existing configuration clickthen select it from the list box and confirm thedeletion with Ok.Settings for multiple track configurations in Channel ModeMultiple track set-ups for sequential scanning can be defined as one configuration (Channel ModeConfiguration), to be stored under any name, reloaded or deleted.The maximum of four tracks with up to eight channels can be defined simultaneously and then scannedone after the other. Each track is a separate unit and can be configured independently from the othertracks with regard to channels, Acousto-Optical Tunable Filters (AOTF), emission filters and dichroic beamsplitters.The following functions are available in the List of Tracks panel in the Imaging Setup Tool (Fig. 17,Fig. 18 and Fig. 19).Switch track everyLine Tracks are switched during scanning line-by-line. The following settings can be changedbetween tracks: Laser line, laser intensity and channels.Frame Tracks are switched during scanning frame-by-frame. The following settings can bechanged between tracks: Laser line and intensity, all filters and beam splitters, thechannels incl. settings for gain and offset and the pinhole position and diameter.Frame Fast The scanning procedure can be made faster. Only the laser line intensity and theAmplifier Offset are switched, but no other hardware components. The tracks are allmatched to the current track with regard to emission filter, dichroic beam splitter,setting of Detector Gain, pinhole position and diameter. When the Line button isselected, the same rules apply as for Frame Fast.Add Track buttonAn additional track is added to the configuration list in the Imaging SetupTool. The maximum of four tracks can be used. One track each with basicconfiguration is added, i.e.: Ch 1 channel is activated, all laser lines areswitched off, emission filters and dichroic beam splitters are set in accordancewith the last configuration used.Remove button The track marked in the List of Tracks panel is deleted.A click on this arrow button will move the selected track (highlighted in lightgrey) one position upwards in the list box.A click on this arrow button will move the selected track (highlighted in lightgrey) one position downwards in the list box.Fig. 19 Track Configurations window