04/2009 5Introduction to ZEN – Efficient NavigationThe ZEN 2009 interface is clearly structured and follows the typical workflow of the experimentsperformed with confocal microscopy systems:On the Left Tool Area (Fig. 4/D) the user finds the tools for sample observation, image acquisition,image processing and system maintenance, easily accessible via four Main Tabs (Fig. 5/1). All functionsneeded to control the microscope can be found on the Ocular Tab, to acquire images use theAcquisition Tools (Fig. 5/3 and 4). Arranged from top to bottom they follow the logic of theexperimental workflow. The area for viewing and interacting with images is centered in the middle of theMain Application Window: the Center Screen Area. Each displayed image can be displayed and/oranalyzed with many view options available through view tabs which can be found on the left side of theimage. According to the chosen view tab, the required view controls appear in View Control Tabs beloweach image. File management and data handling tools are found in the Right Tool Area (see Fig. 4 andFig. 5).Color and brightness of the interface have been carefully adjusted to the typical light conditions of theimaging laboratory, guaranteeing optimal display contrast and minimal stray light for high-sensitivitydetection experiments. The ZEN software is optimized for a 30" TFT monitor but can also be used withdual-20" TFT setups.A focus in the development of ZEN 2009 was to fulfill the needs of both basic users and microscopyspecialists. Both types of users will appreciate the set of intuitive tools designed to make the use of aconfocal microscope from Carl Zeiss easy and fast:The Show all concept ensures that tool panels are never more complex than needed. With Show all de-activated, the most commonly used tools are displayed. For each tool, the user can activate Show allmode to display and use additional functionality (Fig. 6).Fig. 6 Show all mode