Zeiss Standard 25 ICS Operating Manual

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Zeiss Standard 25 ICS Operating Manual Manual pdf 37 page image

Standard 25 ICSB 40-029 e 01/98 3-93.5 Setting of transmitted-light polarization contrastAs described in chapter 2, the microscope is ready for operation and switched on according to section3.1.Transmitted-light polarization contrast requires thefollowing equipment:- polarizer, oriented in EAST-WEST direction,(453615) (3-9/3), mounted on swing-outcarrier.- analyzer (3-9/1), oriented in NORTH-SOUTHdirection, and lambda plate (3-9/2) aremounted on two separate sliders (453692) andintegrated into the stand head. To enable thefunction to be performed, the sliders must bepushed in.Settings: First, set a suitable object in brightfield asdescribed in section 3.2. Swing the polarizer (3-9/3) in the beam path. If only the analyzer slider (3-9/1) is pushed in,black-and-white polarization contrast isproduced; the additional insertion of thelambda plate (3-9/2) makes the objects appearin colored polarization contrast.The sliders containing the analyzer and the lambda plate must always be pushed in until stopto make them fully effective and to avoid the field if view to be cut off.Retrofitting of the polarization equipment can be performed by the Zeiss service agency (seesection 4.4).Fig. 3-9 Setting of polarization contrast