Operating Instructions — Tune and Calibrate4500 Series of Instruments System User Guide50 of 138 RUO-IDV-05-0264-A8. Select a Tuning Solution.Depending on the solution selected, different modes are available.i. Click a polarity.ii. If available, click Q1 and Q3 in the Quad section. If available, click therequired scan speeds.iii. If available, click the scan speeds in the LIT section.iv. If available, click Excitation in the MS/MS/MS section.9. Click Next.10. If the Select a mode page opens, select Automatic.11. Click Next.12. Click GO.The Verifying or Adjusting Performance screen opens. After the process hascompleted, the Results Summary opens. For more information, refer to the Help.13. If applicable (depending on the options selected), change solutions when prompted.Verify or Adjust PerformanceThe top left corner shows the part of the instrument that is being tuned.Current Spectrum: This graph shows the spectrum of the current scan, the optimal scanselected by the software, or the scan at the current parameter value when the software resultsare viewed in interactive mode.The Instrument Optimization Decision Plots, in the top right graph, dynamically show theintensity versus voltage curves of the parameters that are currently being optimized.Results SummaryThe Results Summary is a record of any instrument settings changes that were made by theInstrument Optimization software. Refer to the Results Summary shown in Figure 7-1 onpage 51. This includes the location of data files and instrument settings backups, as well as step-by-step changes and results during optimization.The Results Summary also shows averification report. This report contains a snapshot of the mass spectrum for each relevant massfor the scan modes being verified. The spectrum is labelled with the target mass, where the masswas found, mass shift, peak width, and peak intensity. The spectrum can be used as a visualrecord of peak shape or scan mode performance. A summary table of results follows the spectra.The Results Summary is saved as a document in the folder indicated at the top of the report.Users can print the Results Summary or open a previously saved Results Summary.
