Azure c150, c200, c300, c400, c500, c600 User Manual
                   | |
Contents
| 
Azure™ cSeries User ManualPage 113.3 A Imaging Workflow for Chemiluminescent Applications1. Remove the Blot Tray from the cSeries door.2. After you have applied the substrate to your blot, place your blot in the very center of the tray.3. Place the blot tray in one of two positions: on the UV transilluminator, or on the chemi shelf, inthe middle of the imaging system.• Use the position on the UV transilluminator for larger blots.• Use the position on the chemi shelf for small blots. Using this top shelf increases theresolution of the blot.4. Close the door to the system.5. Select an exposure time. Choose the exposure time manually or use autoexposure which willallow the software to capture image for you using the full dynamic range of the camera.• Recommendation: Set the exposure time to 30 Seconds by hitting 3 then 0 and then S.Keep the Sensitivity setting on the default setting, NORMAL. Select CUMULATIVE. After hittingCAPTURE, a series of images will be displayed, and you can select the best one from thegallery tab.• Maximum exposure time is 60 minutes.6. Select CAPTURE.7. The images will be displayed in the gallery tab.3.4 RGB Applications (c400 and c600)Selecting RBG will give you the follow screen:The RGB setting is for fluorescent Western blots labeled withCy5/Cy3/Cy2 or dyes with similar excitation and emissionspectrums.For all RBG blots, use the Blot Tray on the UV transilluminatorposition only. The blot tray provides a dark background toimprove the contrast on the blot image. Placing this Tray onthe UV transilluminator position provides even illuminationfor your samples.Positions for blot tray:Chemi Shelf (for chemi blots only)UV Transilluminator (for all blots) |
Related manuals for Azure c150, c200, c300, c400, c500, c600
This manual is suitable for:
manualsdatabase
Your AI-powered manual search engine