Azure c150, c200, c300, c400, c500, c600 User Manual
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Contents
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Azure™ cSeries User ManualPage 12For each wavelength, you can set a unique exposure time. Each channel will be captured in a uniquecolor channel, and will be displayed in a multicolor overlayed image. You can take a 1, 2 or 3 channelimage.1. Place blot in the center of the Blot Tray.2. Close the door.3 A. Manual Exposure:• Touch the exposure time box next to the channel that you want toimage. Type in an exposure time using the keypad.See section 3.2A on how to use the keypad.• To take a multi channel image, such as a Cy5/Cy3 image, enter anexposure time for both the Cy5 and Cy3 channel.• For all channels you do not want to image, leave the exposure time at 0.• Maximum exposure time is 5 minutes per channel.3 B. Auto exposure: autoexpose will calculate the best imaging time for you image. After selectingAUTOEXPOSE, simply press CAPTURE. You can choose auto exposure for all, one, or twochannels.4. Select the resolution. The higher the resolution, the longer we recommend setting yourexposure time.5. Select CAPTURE. The channels will be captured sequentially.6. The resulting image will be displayed in the gallery tab.3.5 NIR Applications (c500 and c600)For all NIR blots, use the Blot Tray on the UV transilluminatorposition only. The blot tray provides a dark background toimprove the contrast on the blot image. Placing this Tray onthe UV transilluminator position provides even illuminationfor your samples.1. Place blot in the center of the Blot Tray on the UVtransilluminator position.2. Close the door3 A. Manual Exposure:• Touch the exposure time box next to the channel that you want toimage. Type in an exposure time using the keypad.See section 3.2A on how to use the keypad.• To take a multi channel image, enter an exposure time for both the 700 and 800 channel.• For all channels you do not want to image, leave the exposure time at 0.• Maximum exposure time is 5 minutes per channel.3 B. Auto exposure: autoexpose will calculate the best imaging time for you image. After selectingAUTOEXPOSE, simply press CAPTURE. You can choose auto exposure for all, one, or twochannels.4. Select the resolution. The higher the resolution, the longer we recommend setting yourexposure time. |
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