14Single Image Acquisition or Visualization1. Go to the Locate tab2. In the Favorites section, select the contrast method or filter set you would liketo acquire or visualizeThe FL off mode activates the transmitted light and closes the shutter of incidentlight (switching off fluorescence optics). To fully activate the transmitted light, youshould also open the diaphragm slide until the rear stop (in the Transillumination base300), so that a large homogeneous luminous field can be obtained.