32Downstream Image Processing and AnalysisA free software license is available for ZEN lite at ZEISS website(https://www.zeiss.com/microscopy/int/products/microscope-software/zen-lite.html). Installand use such version in your own personal computer for downstream image analysisOpen1. Go to File -> Open2. Search for the .czi files previously saved in the directory dialog3. The selected files will be displayed in the Centre Screen Area and Right Tool AreaDisplay Adjustments1. In the Dimensions tab of the Centre Screen Area, activate the Range Indicatorcheckbox. This will mark in red or blue overexposed (too bright) or underexposed (toodark) areas, respectively