Zeiss LSM 510 Brief Operating Manual

Also see for LSM 510 Inverted: Quick guide Operating manual Operating manual User guide User manual

Contents

07/05 7Selecting an objective• Open the graphical pop-up menu by clicking onthe Objective button (Fig. 10).• Click on the objective you want to select. Theselected objective will automatically move intothe beam path.Focussing the microscope for transmitted light• Open the graphical pop-up menu by clicking onthe Transmitted Light button (Fig. 11).• Click on the On button. Set the intensity of theHalogen illuminator using the slider.• Click on Close to close the pop-up menu.• Place specimen on microscope stage. The coverslip must be facing up.• Use the focusing drive of the microscope tofocus the required object plane.• Select specimen detail by moving the stage in Xand Y using the XY stage fine motion control.Setting the microscope for reflected light• Click on the Reflected Light button to openthe shutter of the HBO 100 mercury lamp.• Click on the Reflector button and select thedesired filter set by clicking on it.Storing the microscope settingsMicroscope settings can be stored and up to 8buttons assigned for fast retrieval and adjustmentusing the Microscope Settings panel.The Store button permits existing microscopeconfigurations to be stored under any name.The Apply button permits existing storedmicroscope configurations to be loaded.The Delete button permits existing microscopeconfigurations to be deleted.The Assign button permits the assignment of amicroscope configuration to a button.Note: Depending on the microscopeconfiguration, settings must be done manually ifnecessary.Fig. 10 Microscope Control window,e.g.: Axiovert 200 MFig. 11 Microscope Control window withTransmitted Light pop-up menu