Zeiss LSM 710 SIM Manual/quick Manual

Saving/exportingData is not automatically saved after acquisition, it is only stored in the memory. It isrecommended to save always after every acquisition. If an unsaved data is visible in the imagewindow and Live/Snap/Start experiment is pushed, the unsaved data will be overwritten.Check BioVis guidelines where to save/export your data. Saving anywhere else is not allowedand all data will be removed from unauthorized locations.There are two ways of saving:• Save the entire data with all the settings. It is recommended to always save the fulldata first. Later if the data file reopened, all the imaging settings can be reused by theReuse button. Note the data file is not an image file so it cannot be opened in allimaging software.To save the data file, go to File\Save as\ , select the saving location, give a name to thefile, and save the data file as .czi or .lsm (LSM5) file.• Export an image file of your dataset. This you can open and modify in imagingsoftware and send for publication. The recommended file is .tif (Tagged image file).To export a .tif file, go to File\Export\ , select .tif format and under the data pickcontents of the image window. This will save what you see in the image window. It ispossible to take away undesired channels or add scale bars and export that way. AlsoSplit mode or Orthogonal mode can be exported as an image. If Z-stacks or timelapse was done, it is possible to export all the stacks as separate .tif files, for this gofor Contents of the image window – series.