Zeiss LSM 710 SIM Manual/quick Manual

Acquisition tab, SIM imagingHere is only the SIM imaging showed, for a detailed description of the ZEN Acquisition tab,please check first the “Acquisition tab, confocal” part.Grid rotation for SIMimaging, use 5 rotation forthe best resultSelect SIM imaging modeSelecting the emissionfilter for the track (OBS!Exclude FSet77 HE, as thisfilter is for the fluorescentlamp and eyepieceSelecting excitation laserfor SIM (Elyra)Resolution, quality andcropping of the imaging,make no change hereSelect the desired tracknumberWhen laser selected, thecorrect grid is picked up bythe softwareBe sure you have selectedthe 63x/1.4 objective forSIM imagingAdd a color to the trackBe sure Frame or Z-stack isselected if more tracks existChange the laser power to getthe correct intensity (see nextpage)Exposure time for the camera,usually no need to change it,try to keep it on 100 ms as adefault