tecan sunrise Instructions For Use Manual
Also see for sunrise: Instructions for useOperating manual
                                                                                    | |
Contents
| 
TECAN 7. Quality Control2006-03 Instructions for Use for sunrise Absorbance Reader No. 30008746 Rev. No. 1.4 7-97.3.5 Instrument Linearity with Liquid SamplesThe linearity for the instrument and application at the wavelength used can bechecked by using a dilution series of a solution.The result depends on the purity of the dye used and the meniscus of the liquid inthe wells.As a reference, dilution series of Methyl Orange in 0.1 % Tween 20 solution formeasurements at 492 nm can be used.The dilution series should be within the range of 0.1 to 3.0 OD for a standard or6-filter instrument and within the range of 0.1 to 2.5 OD for a tuneable wavelengthinstrument.For other wavelengths, different solutions must be used.250 ml of each dilution are then pipetted into the microplate, a minimum of atleast two samples should be used for each dilution to reduce the errors causedby pipetting.Make sure that the instrument is using the accuracy measurement mode.The microplate is then measured and a linear regression of OD againstconcentration is drawn from the average of the measured OD values.Determine the unweighted residual square value R 2 of the regression line.Typical residual square values for a standard application are equal or better thanR 2 = 0.998.NoteData can vary due to pipetting inaccuracy, meniscus of the liquid and theapplication used.NoteThe limits of the instrument’s linearity will be measured with the QC plate.Example for a tuneable wavelength instrumentReadings 0.100 to 2.500 ODDilution Factor Absorbance1 2.621 OD0.5 1.323 OD0.25 0.679 OD0.125 0.360 OD0.0625 0.192 OD0.03125 0.110 OD0 0.025 ODEquation for linear regression: y = 2.5888x + 0.0314Residual squared value: R 2 = 1 |
Related manuals for tecan sunrise
This manual is suitable for:
manualsdatabase
Your AI-powered manual search engine