6-10 Echo Liquid Handler User Manual6.2.3 Clean and refill the coupling fluid bottleClean and refill the fluid coupling bottle when one of the followingoccurs: Fluid appears cloudy. Two weeks have passed since the last coupling fluid refill. Fluid transfer accuracy or precision has been affected by couplingfluid contamination.Note: During periods of heavy duty cycle use, refilling the couplingfluid bottle will be necessary more frequently than once every twoweeks. It is acceptable to skip the complete fluidic circuit purge as longas it is performed at least every second week.The following procedures are needed to clean and refill the coupling fluidbottle.1. Empty the coupling fluid bottle2. Purge the fluid circuit3. Clean and refill the coupling fluid bottle4. Cycle fluid through the Echo systemThis section describes each task in detail.Tools and materials needed 4 liters of distilled water. The Echo fluidic system holds about1100 mL of fluid (approximately 225 mL in the fluidic circuit and875 mL in the coupling fluid bottle). Emptying, purging, and fillingthe system will be performed several times.Caution: Do not use de-ionized water, as it may corrode the fluidicssystem. An empty, clean 1000 mL coupling fluid bottle (square bottle catalognumber ECHO-AL15-S; round bottle catalog number ECHO-AL-15-R) 50 μL of algaecide (Labcyte catalog number ECHO-AL04) Gloves for protection when handling the algaecide Paper towels or shop cloths to deal with any unexpected fluid leaks Screwdriver for removing the fuse coverThe fluidic system will be emptied, purged, and refilled several times,therefore take note of the following caution:Caution: When working with the Echo coupling fluid system andturning its coupling fluid pump on and off, make sure that thevacuum line is connected and working before turning the couplingfluid pump back on. If the vacuum line is disconnected or the vacuum
