1-4 Echo Liquid Handler User Manual1.1.2 Will the fluid splash or fall out?The Echo liquid handler performs liquid transfer with an inverteddestination plate. Users might question whether all of the droplet remainsin the destination plate well after transfer. Will any of it splash out? Will therepeated motions of the destination plate, or simply time passing, allowany of the transferred liquid to fall or drain out of the destination platewell?Pre-loaded 1536-well destination microplates will not experience fluid losswhen they are inverted. Pre-loaded 384-well destination microplatescontaining fluid with low surface tension may experience fluid loss wheninverted and should be tested. Pre-loaded 96-well destination microplatesmay experience loss of fluid when inverted, depending on fluid type andvolume, and are not recommended for “wet” (pre-loaded) fluid transfers.